Dr. Azizollah Bakhtari, MD is the Chairman of the WAMS Council of Iran.

Education

• Doctor of Philosophy in Animal Genetics and Breeding. 2009-2014.
• Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.GPA: 19.02/20
• Topic: Effect of Mouse Embryo Vitrification on Expression of Methylation Modifiers and Imprinted Genes Advisors: H. R. Rahmani and M. H. Nasr-Esfahani
• Sabbatical in Department of Animal Sciences, University of California Davis, CA, USA. 2013 (6 months).
• Topic Effects of knockdown of DPPA3 in germinal vesicle (GV) stage oocytes on epigenetic changes of resultant embryos Advisors: P. J. Ross
• Master of Science in Animal Genetics and Breeding. 2006–2008.
• College of Agriculture, Department of Animal Sciences, Isfahan University of Technology, Isfahan, Iran.GPA: 17.82/20
• Topic: IGF-IR Polymorphism Gene and its Relation with Holstein and Najdi Dairy Cow’s Performance and Productive Traits Advisors: H. R. Rahmani and M. A. Edriss
• Bachelor of Science in Animal Sciences. 2002–2006.
• College of Agriculture, Department of Animal Sciences, Yasuj University, Yasuj, Iran.
• GPA: 18.05/20

Publications
Peer-reviewed papers
ISI papers

⦁ Bakhtari A. and Ross P. J. 2014. DPPA3 prevents cytosine hydroxymethylation of the maternal pronucleus and is required for normal development in bovine embryos. Epigenetics 9: 1271-1279.
⦁ Bakhtari A.,Rahmani H. R., Bonakdar E., Jafarpour F., Asgari V., Hosseini S. M., Edriss M. A. and Nasr-Esfahani M. H. 2014. The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst. Cryobiology 69: 419-427.
⦁ Bonakdar E., Edriss M. A., Bakhtari A.,Jafarpour F., Asgari V., Sadeghi-Boroujeni N., Hosseini S. M., Rahmani H. R. and Nasr-Esfahani M. H. 2015. A physiological, rather than superovulated, postimplantation environment can attenuate the compromising effect of ART on gene expression of developing mice embryos.Mol. Reprod. Dev. 82:191-206.
⦁ Omidi M., Niknahad H., Noorafshan A., Fardid R., Nadimi E., Naderi S., Bakhtari A., Mohammadi-Bardbori A. 2018. Atypical co-activation of aryl hydrocarbon receptor and Wnt/β-catenin signaling pathways induces cardiovascular developmental abnormalities in mice. Biological Trace Element Research.
⦁ Bakhtari A.,Rahmani H. R., Edriss M. A. and Sayed Tabatabaei B. E.2010. Association of bovine insulin-like growth factor-I receptor polymorphism gene with milk production traits in Iranian Najdi and Holstein dairy cows. Cuban J.Agr.Sci. 44 (4): 343-346.
⦁ Omidi M., Niknahad H., Noorafshan A., Fardid R., Nadimi E., Naderi S., Bakhtari A.,Mohammadi-Bardbori A. Atypical co-activation of aryl hydrocarbon receptor and Wnt/β-catenin signaling pathways induces cardiovascular developmental abnormalities in mice. Biol. Trace Elem. Res. (accepted).
⦁ Mehrsorosh H., Gavanji S., Larki B., Mohammadi M.D., Karbasiun A., Bakhtari A.,Hashemzadeh F. and Mojiri A. 2014. Essential oil composition and antimicrobial screening of some Iranian herbal plants on PectobacteriumCarotovorum. Global NEST J. 16: 239-250.
⦁ Gavanji S., Larki B. and Bakhtari A. 2015. Comparative analysis of extract of Punica granatum var. pleniflora (Golnar-e-farsi) to some antibiotics on pathogens. J. Essent. Oil Bear. Pl. 18:168-178.
⦁ Gavanji S., Larki B. and Bakhtari A. 2014. The effect of extract of Punica granatum var.pleniflora for treatment of Minor Recurrent Aphthous Stomatitis (MiRAS). Integr. Med. Res. 3:83-90.
⦁ Gavanji S., Mohammadi E., Larki B. and Bakhtari A. 2015. Chemical composition identification and comparison antibacterial activity of some essential oils against three pathogenic bacteria. IJSRK. 3:94-105.
⦁ Gavanji S., Mohammadi E., Larki B. and Bakhtari A. 2014. Antimicrobial and Cytotoxic evaluation of some herbal Essential oils in comparison with common Antibiotics in Bioassay condition. Integr. Med. Res. 3:142-152.
⦁ Gavanji S., Sayedipour S.S., Larki B. and Bakhtari A. 2015. Antiviral activity of some plant oils against herpes simplex virus type 1 in Vero cell culture. J. Acute Med. 5:62-68.
⦁ Gavanji S., Zaker S. R., Golestannejad Z., Bakhtari A.,Shirani-Bidabadi E. and Larki B. 2015. Comparative Efficacy of Herbal Essential oils with Amphotricin B and Ketoconazole on Candida albicansin vitro condition. Integr. Med. Res. 4:112-118.
⦁ Kgozeimeh F., Golestannejad Z., Tofighi M., Ayen A., Doost-Mohammadi M., Gavanji S. and Bakhtari A. 2014. Antibacterial activity of milk vetch flower honey against four bacteria of Human oral flora: Streptococcus mutans, Lactobacillus casei, Lactobasillusrhamnosus and Lactobasillusplantarum. Ann. Res. Rev. Biol. 4:3335-3344.
Under review ISI papers
⦁ Bahmanpour S., Bakhtari A.,Abouhamzeh B. Protective Effects of Supplemented Vitrified-Warmed Mouse Oocytes Media with Clove Buds (Syzygiumaromaticum) Extract: A Comparison with α-tocopherol. Cryoletters.
⦁ Salehi M., BakhtariA.,AbouhamzehB., Zare Z.,Hosseini A. Epigenetic changes of nuclear transfer embryos derived from bovine adipose tissue-derived stem cells (BADSCs) compared with in vitro fertilized and parthenogenetic embryos. In Vitro Cellular & Developmental Biology-Animal.

Other papers

⦁ Bakhtari, A.,Rahmani, H.R. 2014. Effect of sperm concentration and condition of in vitro maturation and fertilization of oocytes of under heat stress bovine on polyspermy in derived zygotes. Iranian Journal of Veterinary Clinical Sciences. 7: 53-62.
⦁ Bonakdar, E., Edriss, M.A., Bakhtari, A.,Jafarpour, F., Asgari, V., Hosseini, S.M., Hajian, M., Nasr-Esfahani, M.H. 2014. Comparison of the efficacy of two culture media, handmade (SOF) and commercial (G1/G2), on in vitro development of vitrified and non-vitrified mouse embryos. Iranian Journal of Veterinary Clinical Sciences. 8: 15-23.
⦁ Bahmanpour S., NamavarJahromi B., Koohpeyma F., Keshavarz M., Bakhtari A.2017. Effects of different doses and time dependency of busulfan on testes parameters and spermatogenesis: A quantitative stereological study. Journal of Advanced Medical Sciences and Applied Technologies. 3: 3.
Book publication
⦁ Edriss M.A, Bakhtari A., Mirzaee M., Hashemzadeh F. and Adami B. 2011. Animal Science book: preparing for master of science exame (Volume3 & 4). Daneshpajoohane barin
⦁ Edriss, M.A, Bakhtari A. and Zahmatkesh A. 2011. Statistical analysis and interpratatation methods in seiences. (Translation & Compilation).

Conference attended
Poster presentations
International Congress

⦁ Bakhtari A., Jafarpour F., Asgari V., Bonakdar E., Hosseini S. M., Hajian M., Rahmani H. R. and Nasr-Esfahani M. H. 2012. Mouse embryo vitrification cannot effect on global DNA methylation in preimplantation. IJFS, V. 6, sup. 1, P:82.
⦁ Bakhtari A., Rahmani H. R., Edriss M. A. and Sayed Tabatabaei B. E. 2009. IGF-IR gene polymorphism of Iranian Holstein cows in Isfahan province. ASAS-CAAV Pacific Rim Conference. P: 67.
⦁ Bakhtari A., Rahmani H. R., Jafarpour F., Asgari V., Bonakdar E., Hosseini S. M., Hajian M. and Nasr-Esfahani M. H. 2013. Assisted reproductive technologies interfere with normal DNA methylation profile at Blastocyst stage. The second international conference on agriculture and natural resources, Kermanshah, Iran, P:36.
⦁ Bonakdar E., Asgari V., Jafarpour F., Bakhtari A., Hosseini S. M., Edris M. A. and Nasr-Esfahani M. H. 2013. Aberrant gene expression profile in blastocyst embryos as consequences for assisted reproductive technologies. IJFS, V. 7, sup. 1, P:197.
⦁ Bonakdar E., Asgari V., Jafarpour F., Bakhtari A., Hosseini S. M., Hajian M., Rahmani H. R., Edris M. A. and Nasr Esfahani M. H. 2012. Effect of Mouse Embryo Vitrification on Histone Modifications. IJFS, V. 6, sup. 1, P:83.
⦁ Jafarpour F., Asgari V., Bakhtari A., Bonakdar E., Hosseini S. M. and Nasr-Esfahani M. H. 2013. Comparison of the efficacy of two culture media (handmade vs. Commercial) on in vitro development of vitrified and non-vitrified mouse embryos. IJFS, V. 7, sup. 1, P: 72.

National Congress
⦁ Bakhtari A., Rahmani H.R., Edriss M.A. and Sayed-Tabatabaei B.E. 2008. Evaluation of IGF-IR gene polymorphism of Holsteindairy cows inIsfahan province. Third National Animal Science Congress, Mashhad, Iran.
⦁ Bakhtari A., Rahmani H.R., Edriss M.A. and Sayed-Tabatabaei B.E. 2008. Evaluation of IGF-IR gene polymorphism of Iranian Najdi cows via PCR-RFLP. National Student Conference of Biotechnology and events, Alzahra University,Tehran, Iran.
⦁ Beigi-Harchegani A., Bakhtari A. 2008. Genetic correlation between productive and reproductive traits in various enveronment. The first National Conference on Livestock and Aquatic Sciences. Sanandaj Islamic Azad University, Sanandaj, Iran.
⦁ Asadollah-purnanaee, H., Edriss M.A., Ansari S., Rahmani H.R., Sayed-Tabatabaei B.E., Javansiah-Bigdilu K., Asgari G.H., Bakhtari A. 2011. Lactoferrin gene polymorphism of Iranian Holstein cows in Isfahan province. First National Congress Of Agricultural Science And Technology, University Of Zanjan, Iran.
⦁ Javansiah-Bigdilu, K., Rahmani H.R., Edriss M.A., Khorvash M., Sayed-Tabatabaei B.E., Reihani-Rostami S.M., Asadollah-purnanaee H., Bakhtari A. 2011. Evaluation of Kappa casein gene polymorphism of Iranian Holstein dairy cows via PCR-RFLP. First National Congress Of Agricultural Science And Technology, University Of Zanjan, Iran.
⦁ Asadollah-purnanaee, H., Edriss M.A., Ansari S., Bakhtari A. 2011. Genetic factors influencing the response to heat stress in dairy cows.The first national seminar on the management of livestock and poultry in tropical areas, Shahid Bahonar University, Kerman, Iran.
⦁ Asadollah-purnanaee, H., Bakhtari A., Ansari S., Edriss M.A. 2011. Biological properties and polymorphism of lactoferrin gene in dairy cows.The Role of Biotechnology in Animal Science, Isfahan University of Technology. Isfahan, Iran.
Ongoing Projects
⦁ Effects of Dextran Coated Superparamagnetic iron oxide nanoparticles on embryo produced by IVF (Finished project).
⦁ Effect of platelet-rich plasma as liquid or gel scaffold on morphological and ultrastructural characteristics of in vitro matured oocyte.
⦁ Evaluation of the potential of fibrin scaffold with decellularized ovarian tissue on the maturation of immature oocytes in mice.
⦁ Effects of adding clove buds extract or vitamin E to mouse vitrified and warmed ovaries media on quality and viability of preantral follicles, oxidative enzyme and apoptotic gene expression of preantral follicles and resultant mature oocytes derived from in vitro culture.

Teaching Experience
• Engineering Statistics and Probability, Bonyan Institute and Education, Shahin-Shahr, Iran.
• Practical section of Poultry Breeding, Isfahan University of Technology, Isfahan, Iran.

Research skills
⦁ Knockdown of gene expression via siRNA injection method.
⦁ RNA extraction, cDNA synthesis, Semi-quantitative RT-PCR, Designing primer, Real-time RT-PCR, PCR-RFLP
⦁ Bisulfite sequencing
⦁ Immunostaining
⦁ Confocal laser scanning microscopy
⦁ Microinjection
⦁ Aspiration of oocyte
⦁ Pre-antral follicle culture in mouse
⦁ In vitro fertilization (IVF), Parthenogenetic activation (PA) and in vitro culture (IVC) of sheep, goat, cow, and mouse
⦁ Zygote and embryo transfer (ET) into oviduct and uterus in mouse
⦁ Surgical for the production of vasectomized mouse
⦁ Ovary, Oocyte and embryo vitrification,
⦁ Differential staining in the blastocyst
⦁ Statistical procedures with SAS, SPSS & Minitab software

Educational achievements and honors

• Ranked as the first student among the four students of Animal Genetics and Breeding in the Ph.D. Graduation
• Ranked as the second student among the forty one students of Animal Sciences in the BSc Graduation
• Member of the Iranian Elites Association

Keynote speaker experiences

• Workshop Lecturer, “A review of methods of genetic evaluation of dairy cattle”, Fourth National
• Conference of Iranian Holstein Association, 18-19 Dec. 2013.

Reviewer of Journals and Congresses

• International Journal of Fertility and Sterility
• Journal of Isfahan Dental College
• Fifth Iranian Congress on Animal Science 2012

References

• H.R. Rahmani, Professor, College of Agriculture, Department of Animal Sciences, Isfahan University of Technology, Isfahan, Iran.
• M.H. Nasr-Esfahani, Professor, Department of Cell and Molecular Biology Royan Institute for Animal, Isfahan, Iran.
• P.J. Ross, Assistant professor, Department of Animal Sciences, University of California Davis, CA, USA.
• M.A. Edriss, Professor, College of Agriculture, Department of Animal Sciences, Isfahan University of Technology, Isfahan, Iran.
• S. Bahmanpour, Professor, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
• S. Alaee, Assistant professor, Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz. Iran.
• B. Abouhamzeh, Associated professor, Department of Anatomical Sciences, Faculty of Medicine AJA University of Medical Sciences, Tehran, Iran.